In principle, it is divided into four areas: reagent preparation area, sample preparation area, amplification area, and amplification product analysis area.

實驗室原則上為試劑準備區、樣品制備區、擴增區和擴增產物分析區四個單獨的工作區域并設有專用走廊，各工作區域應設緩沖間，工作區與緩沖間宜安裝連鎖裝置。不同功能的工作區應是分隔獨立的，各工作區有明顯的標志，不能直通，如果緊密相連，需安裝物品傳遞窗。

In principle, the laboratory consists of four separate working areas: reagent preparation area, sample preparation area, amplification area, and amplification product analysis area, with dedicated corridors. Each working area should have buffer rooms, and interlocking devices should be installed between the working area and buffer room. Work areas with different functions should be separated and independent, with clear signs on each area and cannot be directly connected. If they are closely connected, item transfer windows need to be installed.

實驗室區域的設置并不是一成不變的，以下幾種情況需要說明：

The setting of the laboratory area is not static, and the following situations need to be explained:

如果樣本在擴增之前需要粉碎處理，則需要增加一個區域用于樣品的粉碎。

If the sample needs to be crushed before amplification, an additional area needs to be added for sample crushing.

如果采用實時熒光PCR法，則擴增區和分析區可以合并為一個區。

If real-time fluorescence PCR is used, the amplification region and analysis region can be merged into one region.

如果采用全自動化PCR分析儀，則標本制備區、擴增區和分析區可以合并為一個區。

If a fully automated PCR analyzer is used, the sample preparation area, amplification area, and analysis area can be merged into one area.

前兩區為擴增前區，后兩區為擴增后區，擴增前區與擴增后區應嚴格分開。實驗材料、試劑、記錄紙、筆、清潔材料等，只能從擴增前區流向擴增后區，即從試劑準備區、樣品制備區到擴增區和擴增產物分析區，不得逆向流動。實驗室的氣流也應從擴增前區流向擴增后區，不得逆向流動。各工作區頂部應安裝紫外燈，紫外燈的波長為254nm，每20㎡一支40W的紫外燈。

The first two regions are pre amplification regions, and the last two regions are post amplification regions. The pre amplification and post amplification regions should be strictly separated. Experimental materials, reagents, recording paper, pens, cleaning materials, etc. can only flow from the pre amplification area to the post amplification area, that is, from the reagent preparation area and sample preparation area to the amplification area and amplification product analysis area, and cannot flow in reverse. The airflow in the laboratory should also flow from the pre amplification area to the post amplification area, and should not flow in the opposite direction. UV lights should be installed at the top of each work area, with a wavelength of 254nm and a 40W UV light per 20 square meters.